简介:

Even though isoelectric focusing (IEF) is a very useful technique for sample concentration and separation, it is very challenging to extract separated samples for further processing. Moreover, the continuous sample concentration and separation are not possible in the conventional IEF. To overcome these challenges, free flow isoelectric focusing (FFIEF) can be used in which a flow field is applied in the direction perpendicular to the applied electric field. However, efficient design of microfluidic chip is cumbersome for FFIEF process as one has to take care of both flow and electric field in addition to pH field. In this talk, we will present a new mathematical modelto study the roles of flow and electric fields for efficient design of microfluidic chip for continuous separation of proteins from an initial well mixed solution. Results obtained from our model will be compared with experimental data to demonstrate the efficacy of the model. Finally, a new channel design will be presented to increase the separation resolution by introducing cross-stream flow velocity. Our results indicate that the separation resolution can be improved by three folds in this new design compare to the conventional straight channel design used in traditional experiments.

 

Biography:Prof. Prashanta Dutta received his MS and PhD degrees in Mechanical Engineering from the University of South Carolina and Texas A&M University, respectively. He joined the School of Mechanical and Materials Engineering of Washington State University (WSU) in 2001. His primary research area is Micro, Nano, and Biofluidics with specific focus on the development of new algorithms for multiscale and multiphysics problems. He has published more than 150 peer reviewed journal and conference articles and four book chapters. He chaired numerous sessions, fora, symposia, and tracks for a number of ASME and APS conferences and served as the Chair of ASME Micro/Nano Fluid Dynamics Technical Committee. He is an elected fellow of ASME. He served as an Associate Editor for the ASME Journal of Fluids Engineering. Currently he isa Guest Editor for Electrophoresis.