报告摘要：Metabolic engineering of microorganisms such as Escherichia coli and Saccharomyces cerevisiae to produce high-value natural metabolites is often done through functional reconstitution of long metabolic pathways. Problems arise when parts of pathways require specialized environments or compartments for optimal function. Here we solve this problem through co-culture of multiple engineered organisms, each of which contains the part of the pathway that it is best suited to hosting. In one example, we divided the synthetic pathway for paclitaxel precursors into two modules, expressed in either S. cerevisiae or E. coli, and cultured the microorganisms in the same bioreactor. Stable co-culture was achieved by designing a mutualistic relationship between the two species in which a metabolic intermediate produced by E. coli was used and functionalized by yeast. This synthetic consortium efficiently produced oxygenated taxanes, tanshinone precursors and functionalized sesquiterpenes.