Quantitative and functional proteomics and post-translational modification (PTM) proteomics have emerged as powerful omics approaches in studying cellular events in various model organisms.  In this presentation, I intend to cite a few examples of application of quantitative PTM proteomics in Arabidopsis to show the potential impact of this approach in the plant cell biology research. The first example will be the confirmation of a novel plant growth regulator cis-CA’s role in regulation of gene expression, and the second one will be the discovery of EIN2-independent ethylene signaling in Arabidopsis, in which the time-dependent and dual-and-opposing (DOE) effect of ethylene was investigated using the stable isotope metabolic labeling (SIML)-based quantitative phosphoproteomics performed on various hormone-signaling component mutants. Those cell-signaling mutants include those deficient in either a kinase or a phosphatase. This SIML-based quantitative PTM proteomics was also employed to search for rapidly phosphorylated proteins in response to 1-minute of ethylene treatment. Transgenic plant studies of these PTM proteomics-identified candidate proteins revealed that these newly found proteins indeed play a role in phytohermone-mediated cell signaling. Lastly, a novel OxNSIL redox proteomics approach will be introduced to stress its potential application in identification of site-specific thiol redox proteins in both plant and animal systems.