简介: |
Abstract
The cilium is an instrumental sensory organelle that is crucial for sonic hedgehog signalling pathway and is implicated in several other signal transduction pathways. Mutations affecting the structure or function of cilia result in a plethora of diseases, including a number of developmental defects, collectively known as ciliopathies. Localizing and concentrating signalling receptors inside the cilia is a key to its function. How membrane-associated and integrated proteins are selectively localized in cilia is still elusive. Several membrane associated, acylated and prenylated proteins, are targeted to cilia in complex with a family of proteins that share similar structural fold as RhoGDI and act as solubilising factors. We call this family of proteins GDI-like solubilizing factors (GSFs). In my talk I will demonstrate that the G proteins Arl2 and Arl3 act in a GTP-dependent manner as allosteric release factors for farnesylated cargo whereas Arl3 is a specific release factor for myristoylated cargos in a process that is dependent on ciliary localization.
References
1. Structural basis for Arl3-specific release of myristoylated ciliary cargo from
UNC119. Shehab A. Ismail, Yong-Xiang Chen, etc. EMBOJ (AOP 2012)
2. Arl2 and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo. Shehab A. Ismail, Yong-Xiang Chen, etc. Nature Chemical Biology (2011)
3. The GDI-like solubilizing factor PDEδ sustains the spatial organization and signaling of Ras family proteins. Anchal Chandra, Shehab A Ismail, etc. Nature Cell Biology
CV of Shehab Ismail
Shehab Ismail received his B.S. from Cairo University, Egypt and PhD at University of Tennessee/St Jude Children’s Research Hospital, USA. After graduation, he continued his research at College of Medicine, University of Toronto, Canada and Department of Structural Biology, Max-Planck-Institute, Dortmund, Germany as postdoctoral fellow. In 2011, he became the Senior Scientist in the emeritus group Professor Dr. Alfred Wittinghofer in Max-Planck Institute of Molecular Physiology. Dr. Ismail’s research interests include molecular mechanism of membrane trafficking, chemical labeling for visualizing cellular events, chemical genomics, protein modification, protein structure and enzymology. |
