报告摘要：

We report our nucleic acid sensing platform that can identify and enumerate individual short nucleic acids in a heterogeneous sample, with minimum pretreatment.  Although there are broad applications for this highly specific and sensitive high-throughput platform, the preliminary data and the proposed project target Point-of-Care (POC) profiling of a small panel of 22-base microRNAs from human physiological samples. On-chip rapid molecular separation is achieved by flow-electrophoresis with non-uniform field/ionic strength induced by ion-selective membranes.  We also integrate existing conic solid-state nanopore sensing technologies with new nanoparticle(NP) assembly and pore modification technologies to enable high-resolution electro-nanochromatograph separation, molecule-agglutinated nanoaggregate assay, discriminating single-molecule conductance signals, plasmonic-enhanced fluorescence etc for ion-current and nanophotonic sensing. In addition, the intense electro-osmotic and electrophoretic forces at the conic tip are harnessed to minimize non-specific binding.  The result is a sensitive integrated platform that can identify and quantify nearly identical multiple microRNAs with copy numbers between 10 and 10,000, roughly the number in a single exosome released by cellular exocytosis.