报告题目：

Microrobotics and Computer Vision for Biomanipulation

（微型机器人学和计算机视觉技术在生物微操作方面的应用）

报告人：王文慧 博士

报告时间：

11月8日上午10：00~11：00

报告地点：

9003大楼（精仪系系馆）四层大会议室

主办单位：

清华大学精仪系

内容简介：

As important embodiments of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, proteins, and drug compounds) into individual cells, and phenotypic investigation of mutated organisms (e.g., locomotive patterns) have significant implications in genetics, cancer therapy, and drug discovery. This talk will focus on introducing my research in developing microrobotic systems and micro devices for cell mechanical property characterization, fully automated zebrafish embryo injection, semi-automated adherent cell injection, and real-time tracking and feature extraction of C. elegans.

New approaches have been developed to extract cellular force information (sub¬microNewton) and to determine relative depth positions between an end-effector and the micro object to be manipulated down to 0.2µm, both purely based on computer vision microscopy feedback without using extra sensors. The first-of-its-kind autonomous microrobotic injection system is capable of injecting zebrafish embryos at a speed of 15 cells/minute with a success rate of 99%, a survival rate of 98%, and a phenotypic rate of 98.5%. The system has been recently extended to be capable of injecting adherent cells at a speed of 25 cells/minute with a survival rate of 95.7%, and a success rate of 82.4%.

On the organism level, the talk will introduce my collaborative effort in the development of a visually servoed microrobotic system for real-time tracking and extracting locomotive features of the model organism, C. elegans in order to understand how genes control behavioral phenotypes of the nematode (~1mm long, <100µm wide).