材料院《材料科学论坛》:Gradient Approach of Screening Cell-Material Interactions
报告人:
Dr.Murugan Ramalingam
Associate Professor of Université de Strasbourg (UdS), France
报告时间:
2011-12-13 09:30
报告地点:
清华大学材料院学术报告厅（逸夫技术科学楼2－321）
主办单位:
材料院《材料科学论坛》 联系人：王秀梅 老师 62771556 欢迎广大师生踊跃参加
简介:
Abstract:
Biomaterials that have gradients in material composition and functional properties may be used to engineer interfaces between two different tissues (soft-to-hard) or for rapid screening of cell response to scaffold properties suitable for tissue engineering applications. This talk reports a method for generating nanofiber scaffold libraries containing gradients in material composition and properties. The method is based on “bi-spinnerret electrospinning” system, wherein the gradients are obtained by using two syringes where one is filled with poly(ε-caprolactone) (PCL) solution and the other with PCL solution containing calcium phosphate particles, nano hydroxyapatite (nHA) for example. The electrospun scaffolds consisted of non-woven PCL nanofibers containing a linear gradient of nHA, as a single scaffold specimen, which facilitated screening of the effect of nHA composition on human bone marrow stromal cells (hBMSCs) response. The formation of nHA gradients was confirmed by thermogravimetric analysis and their morphology was confirmed by scanning electron microscope. The cellular responses were studied by qualitative (fluoresecence imaging) and quantitative (Picogreen DNA assay) analysis. The percent mass of the nHA present in each pre-determined positions across the scaffold libraries were determined in order to quantify the scaffold composition. Cell attachment (SYTOX green staining) and proliferation (dsDNA quantification) were also examined. The cell culture data showed presence of nHA enhanced the cell adhesion and proliferation compared to pure PCL. In addition, an increased alkaline phosphatase activity has observed on the nHA rich side compared to other end of the scaffold specimen (nHA absence side). These data confirm that the hBMSCs respond to material composition and properties along the graded libraries and can be controlled or tuned their growth and differentiation. The combinatorial screening approach can be utilized to systematically identify scaffold composition and properties that optimize cell responses for tissue engineering applications
CV：
Murugan Ramalingam is an Associate Professor of Biomaterials and Tissue Engineering at the Institut National de la Santé et de la Recherche Médicale(INSERM) U977, Faculté de Chirurgie Dentaire, Université de Strasbourg (UdS), France. Concurrently he holds an Adjunct Associate Professorship at the Tohoku University (Japan). Prior to joining the UdS, he worked as an Assistant Professor at the WPI-Advanced Institute for Materials Research, Japan. He has also worked at the National Institute of Standards and Technology (USA) and National Institutes of Health (USA) under the U.S. National Academies Associateship program. He received his Ph.D. (Biomaterials) from the University of Madras. He has also undergone training in "Ethical and Policy issues on Stem Cells" from the Harvard University, and in "Operations Management" from the University of Illinois-Chicago. His current research interests are focused on the development of multiphase biomaterials, through conventional to nanotechnology to biomimetic approaches, cell patterning, stem cell differentiation and tissue engineering.He has authored over 125 publications, including peer-reviewed journal papers, conference proceedings, book chapters, authored books, edited books, and patents relevant to biomaterials and tissue engineering. He serves on the editorial boards of multiple biomaterials and tissue engineering related journals, including an Editor-in-Chief of “Journal of Bionanoscience” and “Journal of Biomaterials and Tissue Engineering”. He is also a recipient of CSIR fellowship (India), SMF fellowship (Singapore), NRC Fellowship (USA), National Professeur des Universités (France) and Fellow of Royal Society of Chemistry (UK).
