报告题目: |
Laser Cell Micropatterning Technique in Cell-based Biomedical Engineering |
报告人: |
Bruce Z. Gao |
|
Ph.D.
Associated Professor of the Department of Bioengineering,
Clemson University, USA
|
报告时间: |
2011-06-08 15:00 |
报告地点: |
医学科学楼B321 |
主办单位: |
医学院 |
简介: |
The fundamental units of an organism are biological cells, which are involved in the development and maintenance of the hierarchical structures of the organism. Communication between neighboring cells, mediated by physical contact and diffusive signaling, regulate normal cellular functions. The functional characteristics of a single cell are determined by its genetic coding as well as its microenvironment. Due to the extreme complexity of in vivo environments, our understanding of the cellular functions and cell-cell interactions are heavily dependent on cell culture. Numerous studies have demonstrated the importance of cell arrangements for the functions of the cells in culture. To understand the biological mechanisms at cellular level in cell culture, it is necessary to assess the temporospatial interactions of a single cell with its surroundings, including neighboring cells and adjacent extracellular matrix. However, there is no commercially available instrument that can be used to achieve the site- and time-specific placement of an individual cell in a cell culture. There is a critical need to develop a single cell manipulation system for the study of contact and diffusion-mediated communication among neighboring cells. In this talk, I a novel laser cell micromanipulation technique will be described based on the optical force generated by a weakly focused laser beam. In the beam’s focal region, the optical field generates a force with two components: a radial trapping force that draws microparticles, such as cells, into the center of the beam, and an axial pushing force that propels the trapped microparticles along the beam axis and deposits them onto a target surface, a process called laser guidance. Specific cell patterns can be created by moving the target surface relative to the beam axis. Several applications of this technique will be demonstrated, including 1) alignment of adult myocytes, 2) the formation of a neuronal circuit on microelectrode arrays, and 3) laser guidance based cell detection.
|
|
