A Digital Microfluidic Platform for Synthesizing ImmunoPET Tracers
报告人:
Clifton Kwang-Fu Shen
Ph.D. Crump Institute for Molecular Imaging Department of Molecular & Medical Pharmacology, David Geffen School of Medicine at UCLA, USA
报告时间:
2009-10-15 15:30
报告地点:
医学院 B321
主办单位:
清华-霍普金斯生物医学工程联合中心/医学院生物医学工程系
简介:
Biomolecules and their engineered derivatives provide an enormous reservoir for developing highly specific Positron Emission Tomography (PET) imaging probes to visualize biological processes and detect biomarkers of interests in vivo. However, efficient radiolabeling of a delicate biomolecule still remains challenging. Using droplets generated in microfluidics, which resemble isolated, miniature batch microreactors composed of different reagents and compositions, enables large-scale screening and exerts an unprecedented degree of control over individual reaction conditions. We have developed a novel microfluidic chip capable of generating oil-free droplets and manipulating minute volume of liquids. The feasibility of on-chip radiolabeling of biomolecules was demonstrated using various biomolecules and a common 18F-tag, N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB). Consumed only small amount of precious biomolecules, the optimal reaction parameters can be obtained by screening many individual droplets (analytical-scale “batches”) with different compositions. Batches of products can be generated from one single droplet to mouse-dose scale to match the demand of radioactivity for radiotracer evaluation. Data from in vitro studies and in vivo micro-PET scans using 18F-labeled engineered antibody fragments (diabodies) will be presented.
