摘要：

Understanding of cell activities often requires live fluorescence imaging with high spatial and temporal resolution. However, live fluorescence imaging is challenging, due to tradeoffs between spatial resolution, imaging speed, photobleaching and photodamage. Here, we report a live fluorescence imaging technique, Bessel beam plane illumination microscopy, with spatial resolution beyond the diffraction limit and temporal resolution at the level of seconds per 3D volume, while only induces low photobleaching and photodamage. The method combines planar illumination produced by scanned Bessel beams with super-resolution structured illumination microscopy. We demonstrate high speed super-resolution imaging with examples from single cell to multicellular length scales. In comparison with widely adopted spinning-disk confocal and other live super-resolution imaging techniques on samples at different scales, Bessel beam plane illumination microscopy shows significant advantages in balancing spatial resolution, imaging speed, photobleaching and photodamage for live fluorescence imaging.

报告人简介： 

Liang Gao received his Ph. D. from the department of Chemistry at Purdue University with Dr. R. Graham Cooks in 2009. His Ph.D. research was focused on the development of miniature mass spectrometers. He did his postdoctoral research in the Howard Hughes Medical Institute at the Janelia Farm Research Campus with Dr. Eric Betzig, working on high speed, high resolution imaging techniques to advance the fundamental research of life sciences. He is now an assistant professor of the department of Chemistry and the department of Biochemistry and Cell Biology at the State University of New York at Stony Brook. In recent five years, Dr. Gao has published more than ten refereed journal papers, and five of them were in Science, Cell, Nature Methods, and PNAS.